Join via Zoom at https://usc.zoom.us/j/96942386706?pwd=MCtGZ0FpUzVOS0tWMmkxekt0M2N5QT09

1 pm
“A spatial transcriptomic view of injury-directed repair of the mammalian kidney”
Andy McMahon, PhD, and Long Cai, PhD
Presenter: Louisa Gerhardt, PhD

Acute kidney injury, triggered by a variety of insults, leads to the death of epithelial proximal tubules and the activation of a reparative response by surviving proximal tubule cells. Injury repair occurs in close association with infiltrating immune cell types and impacts vascular and interstitial cell types in the reparative niche. We have explored the potential of seq-FISH, a single cell, spatial transcriptomic approach developed in the Cai laboratory (Caltech), to generate a spatially resolved understanding of cell interactions underlying successful and unsuccessful renal repair processes.

1:20 pm
“Mapping the spatio-lineage organization of hematopoietic stem and progenitor cells in the bone marrow”
Rong Lu, PhD, and Michael Elowitz, PhD
Presenters: Martin Tran and Basia Gala

Hematopoiesis depends on stem and progenitor cells that proliferate, move, and differentiate within the bone marrow. The spatial arrangements and lineage relationships among these cells play crucial roles in controlling fate choices, but remain largely uncharacterized. We are therefore adapting the genomic recording technique called MEMOIR to map the states, lineage relationships, and spatial organization of these cells directly within bone marrow in transplantation and transgenic mouse models.

1:40 pm
“Transcriptome-function relationships underlying disease in the adult human brain”
Michael Bonaguidi, PhD, Robert Chow, MD, PhD, and Jonathan Russin, MD
Presenter: Victoria Wolseley

Epilepsy is a very common neurological disorder presenting seizures and cognitive comorbidities. Surgical resections from adult epilepsy patients provide a rare opportunity to investigate how endogenous neural stem cells and their niche cells contribute to syndromic phenotypes. I will provide an update on our progress using “Patch-Seq” that links single cell transcriptomes to function, with a focus on newborn human astroglia.