Tuesday, September 28, 2021 at 11:00am to 12:00pm
To register for this Zoom seminar, please visit https://usc.zoom.us/meeting/register/tJAqdOmvrDwiHdZyplxWIV0xNNCRq_5LNYNp
Our goal is to understand mechanisms of development and integration of the nervous, vascular and hepatic systems, with a focus on Notch signaling and Alagille syndrome. In order to rapidly and flexibly perform perturbations in mouse embryos, we developed neural plate targeting with in utero nano-injection (NEPTUNE) to manipulate gene expression in the developing nervous system with high spatio-temporal control. Next, in order to resolve and quantify the architectural mechanisms contributing to phenotypic recovery in Alagille syndrome, we developed double resin casting micro CT (DUCT), allowing us to quantitatively define liver architecture in mouse models. With these tools in hand, we are now performing lineage tracing and high through-put perturbations to investigate mechanisms of development controlled by Notch.
Host: Nils Lindstrom