USC Stem Cell Seminar: Can Cenik, PhD, University of Texas at Austin- "Single cell quantification of ribosome occupancy and translation in development"

Tuesday, November 1, 2022 at 11:00am to 12:00pm

This is a past event.

Broad CIRM Center (BCC), BCC- 101
1425 San Pablo Street, Los Angeles, CA 90033

In-person or Register via Zoom at https://usc.zoom.us/meeting/register/tJAqdOmvrDwiHdZyplxWIV0xNNCRq_5LNYNp

Technological limitations precluded transcriptome-wide analyses of translation at single cell resolution. To solve this challenge, we developed a novel microfluidic isotachophoresis approach, named RIBOsome profiling via IsoTachoPhoresis (Ribo-ITP), and characterized translation in single oocytes and embryos during early mouse development. Our high coverage measurements enabled the first analysis of allele-specific ribosome engagement in early development and led to the discovery of stage-specific differential engagement of zygotic RNAs with ribosomes. The novel Ribo-ITP approach will enable numerous applications by providing high coverage and high resolution ribosome occupancy measurements from ultra-low input samples including single cells.

Department
Stem Cell at USC
Hashtag

#Stemcell

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