Tuesday, March 13, 2018 at 10:30am to 5:30pm
Broad CIRM Center (BCC), First Floor Seminar Room
1425 San Pablo Street, Los Angeles, CA 90033
Webcast link for live viewing: keckmedia.usc.edu/stem-cell-seminar
10:30am: “Molecular recording: Capture and storage of data within the genome of a cell,” Seth Shipman, PhD, Harvard University
Experimental cell biology is limited by the requirement for direct observation or destructive analysis of the cells being studied. This is particularly problematic when studying a complex, developing system such as the brain. To circumvent this limitation, I have pursued a new approach: the logging of biological events as coded nucleotides, written into the genome of a living cell. These organized changes to genomic DNA can store data during an event of interest, to be collected later by sequencing. I will display a functional version of this approach in prokaryotes that uses the CRISPR-Cas adaptive immune system, and discuss opportunities to use this molecular recording system to understand neurodevelopment.
11:45am: "Functional interrogation of lineage infidelity in stem cell fate decisions,” Yejing Ge, PhD, The Rockefeller University
The goal of my research is to understand stem cell plasticity and their ability to rapidly respond to microenvironmental changes by expanding their fate choices to cope with stress and mediate repair. For example, I found that stem cells of the skin undergo “lineage infidelity” to repair wounds, and that cancer can hijack this repair mechanism. In my future lab, I plan to exploit functional genomics tools to study stem cell plasticity in health and in diseases.
2pm: "Engineering natural cell behaviors using optogenetics," Maxwell Z. Wilson, PhD, Princeton University
The ability to manipulate and readout cell state with precision has revealed elaborate, dynamic architectures. From signal transduction to metabolism, a cell’s ability to decide and then execute a given behavior is determined by the organization of molecules at specific times and places. Drawing from work throughout my career, I will demonstrate how coupling powerful experimental approaches that enable precise spatiotemporal manipulation with mathematical modeling has allowed me to uncover strategies through which cells make such decisions.
3:15pm: "Understanding the genetic basis for biological patterning from the bottom up," Pulin Li, PhD, California Institute of Technology
How genes, operating in individual cells, generate coordinated multicellular behavior is a fundamental question in biology. Morphogens, forming concentration gradients in space, set the blueprint for tissue patterning. By reconstituting morphogen gradients in vitro, genetically re-wiring pathway architectures, and using quantitative time-lapse imaging and mathematical modeling, we revealed how tissue patterning dynamics and precision arise from the underlying genetic circuits. The ability to isolate morphogen-mediated patterning from concurrent developmental processes and to compare the behavior of alternative circuit architectures offers a new way to uncover developmental design principles and engineer multicellular patterning.
4:30pm: “New regulatory mechanisms of Wnt and R-spondin signaling in development, stem cells and cancer,” Andres Matias Lebensohn, PhD, Stanford University School of Medicine
The Wnt/R-spondin signaling pathway orchestrates patterning and morphogenesis during embryonic development, promotes tissue homeostasis and regeneration, and contributes to cancer and other diseases when it becomes defective. I will describe several new regulatory mechanisms of Wnt signal transduction uncovered through genetic screens in haploid human cells, a new mode of signaling by R-spondins that does not require their only known cell-surface receptors (LGRs 4-6) and the implications of these discoveries in physiology and disease.
RECEPTION TO FOLLOW