Tuesday, January 29 at 12:00am to 1:00pm
Michelson Center for Convergent Bioscience (MCB), 101
1002 West Childs Way Los Angeles, CA, 90089
Dopamine (DA) is a central monoamine neurotransmitter involved in many physiological and pathological processes. A longstanding yet largely unmet goal is to measure DA changes reliably and specifically with high spatiotemporal precision, particularly in animals executing complex behaviors. Here, we report the development of genetically encoded GPCR-activation-based-DA (GRABDA) sensors that enable these measurements. GRABDA sensors can resolve a single-electrical-stimulus-evoked DA release in mouse brain slices and detect endogenous DA release in living flies, fish, and mice. We deploy similar strategies to develop a plethora of GRAB sensors for other important neurotransmitters/neuromodulators.